While examining human sperm in a scanning electron microscope, we got astonished: a spermatozoon bearing an unprecedented anomaly, dramatically recalled the XVII century preformation theory and the theory of "Homunculus".
Neutrophil leucocytes purified from human blood have been made adhere to a glass coverslip and video-recorded upon phase-contrast infrared illumination under a IX70 Olympus microscope.
Leucocytes (white blood cells) purified from human blood are made adhere onto a glass coverslip. Once trasnferred into a special observation chamber kept at 37C, leucocytes are observed with a phase-contrast microscope and nourished with a flow of a physiological solution. Their movements witness the cellular activities
Phase-contrast microscopy: human spermatozoa penetrating cervical mucus in vitro. Once into the cervical mucus, the spermatozoa travel in lines along molecular tracks made of highly hydrated, long glycoproteins. A one-way climbing.
Testicular tubules - they produce, collect and transport spermatozoa - freshly prepared from rat testes contract in response to endothelin (a peptidic hormone) added in vitro. Phase-contrast microscopy
The role of the human sperm acrosome is presented together with an original lab procedure to assess acrosome integrity, important in managing human reproductive problems and in basic research on spermatozoa
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